BSc. Biotechnology II: Enzymology, Immunology & Biostatistics-Mumbai-April 2019


Subject: BSc. Biotechnology II: Enzymology, Immunology & Biostatistics

Semester: 2

[Total Time: 2 ½ Hours]
[Total Marks: 75 ]
N.B.: 1) All questions are compulsory.
2) Figures to the right indicate full marks.
3) Draw a neat labeled diagram wherever necessary.

Q. 1) Do as directed: (any fifteen) (15M)

1) Define: Holoenzyme

2) The class of enzymes involved in oxidation-reduction reactions are ___________.

3) The enzyme _______________ the energy of activation:
a) Increases                b) Decreases

4) Define: Temperature coefficient.

5) Explain the term: Homotropic effects.

6) Give one example of irreversible inhibitors.

7) Enzymes are ______________.
a) Proteins                  b) Carbohydrates

8) What is zymogen?

9) The lymphocyte that produces antibodies _________.
a)  B cell                      b) T cell

10) The immunity in which the T lymphocytes participate is ____________
a) Humoral Immunity.                            b) Cell-Mediated immunity

11) Lysozyme is present in ______________.
a) Tears                                                  b) Sweat

12) A type of traditional vaccine ___________.
a) Live attenuated vaccine                    b) Peptide vaccine

13) Any one secondary lymphoid organ:
a) Bane marrow                                     b) Lymph node

14) Anti Rh antibodies are ________________
a)  Complete antibody.                          b) Incomplete antibodies

15) ___________ is square of standard deviation.

16) Define Range

17) Give the term- Data

18) What is meant by class width?

19) Define-Standard error

20) The values of variables tend to concentrate around the central value and it is the central part of a distribution, they are called as_______________ .

Q. 2) A) Describe the classification of enzymes with example. (8M)

Q. 2) B) Give an account of the various factors affecting enzyme activity. (7M)


Q. 2) C) Explain the mechanism of enzyme action. (8M)

Q. 2) D) With example explain competitive inhibition. (7M)

Q. 3) A) Explain tiny two mechanisms of Innato Immunity. (8M)

Q. 3) B) Explain the structure of an antibody molecule with a neat labeled diagram. (7M)


Q. 3) C) Explain with a diagram the structure of IgA. (8M)

Q. 3) D) Describe Hybridoma technology. (7M)

Q. 4) A) Define-Biostatistics. Discuss the importance of biostatistics in biology. (8M)

Q. 4) B) Explain median and compute for the following data. (7M)

Income (Rs.) 100 150 80 200 250 180  Total
No. of Persons  24 26 16 20 6 30 122


Q. 4) C) Explain representation of data using Pie/Charts Histogram and frequency Curve. (8M)

Q. 4) D) Calculate the mode of the following frequency distribution. (7M)

Height in inches 58 59 60 61 62 63 64 65 66 67 Total
No. of Persons 4 6 5 10 20 22 24 6 2 1 100

Q. 5) Write short note: (Any three)  (15M)

a) Feed-back inhibition.

b) Active site

c) Anyone modern vaccine

d) Anyone precipitation technique.

e) Merits, demerits, and sued of standard deviation.

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