BSc. Biotechnology II: Enzymology, Immunology & Biostatistics | Mumbai-April-2018

MUMBAI UNIVERSITY

Subject: BSc. Biotechnology II: Enzymology, Immunology & Biostatistics

Semester: 2

[Total Time: 2½ hours]
[Total Marks: 75] 
Note: 1 Attempt all questions.
2 All questions carry equal marks.
3  Draw neat labeled diagrams wherever necessary.
4 Use of log tables and non-programmable calculator is allowed.|
5 For Q. 2, Q.3, and Q.4 attempt A and B OR C and D
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Q. 1 Do as directed (ANy fifteen) (15M)

1) Define Zymogen.

2) The functional unit of enzyme is called as _______________
a) apoenzyme         b) holoenzyme          c) co-enzyme

3) Email Fischer proposed the ________ model on an enzyme-substrate interaction.
a) Koshladn            b)  lock and key        c) substrate stain

4) Enzymes reduce the __________ energy to increase the rate o reaction.
a) Binding              b) potential               c) activation

5) ________________ is the non-protein substituent which binds the apoenzymes.
a) cofactor             b) anti-metabolie     c) exzyme

6) The interaction of the substrate with the enzyme causes a conformational change in the enzymes termed is _______________
a) lock and key      b) desaturation       c) induced fit

7 ) ) The substrate binds at the ___________ site on the enzyme.
a) allosteric            b) active                  c) initiation

8 the lymphocyte that produces antibodies.
a) B cell                                       b) T cell

9) The immunity in which the T lymphocyte participate is
a) Humoral Immunity                 b) Cell-mediated Immunity

10) The antibody that does not cross the placental barrier.
a) IgM                                         b) IgG

11) The immunity we obtain on exposure to antigen is
a) Acquired Immunity                 b) Innate Immunity

12) When a particulate antigen reacts with its antibodies to form clamps it is called
a) Aggulation                              b) Precipitation

13) A plant used for edible vaccines.
a)   Potato                                   b) Onion

14) Calculate Median: 2,4,6,8,10,12,14,16,18,20,22.

15) Calculate variance if the standard deviation is 4.

16) Define Mode

17) Define Arithmetic mean.

18) State true or false. A pie chart is a circular statistical graphic which is divided into slices to illustrate numerical proportion.

19) State true or false: Frequency polygon is a smooth curve that corresponds to the limiting case of a histogram computed for a frequency distribution of a continuous distribution as the number of data points becomes very large.

20) Give the formula to calculate Range.

Q. 2) A) Derive the Michaelis-Menten equation. (8M)

Q. 2) B) Elaborate on Enzyme inhibition. (7M)

OR

Q. 2) C) What are enzymes and give an account of different classes of enzymes. (8M)

Q. 2) D) Discuss Allosteric regulation. (7M)

Q. 3) A) Define Aggulations and explain any one technique. (8M)

Q. 3) B) Differentiate between Active and Passive immunity. (7M)

OR

Q. 3) C) Explai with a diagram the structure of IgA (8M)

Q. 3) D) Discuss Hybridoma technology. (7M)

Q. 4) A) What is biostatisteic? Discuss the applications of this branch in biological sciences. (8M)

Q. 4) B) Calculate the arithmetic mean for the following grouped data. (7M)

0-1010-2020-3030-4040-50
510152025

Q. 4) C) Calculate mean and Standard deviation for the marks obtained by 10  students in a  weekly test. (8M)

Roll No.12345678910
1515151518172020131512

Q. 4) D) Explain-Histogram, Pie chart, and frequency curve. (7M)

Q. 5) Write Short notes on any three of the following. (15M)

a) Effect of temperature and pH on enzyme activity.

b Applications of enzymes

c) Features of an antigen.

d) Anyone secondary lymphoid organ

e) Variance and Coefficient of variance.
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